Epipox is a resource customized for epitope vaccine design against pathogenic orthopoxviruses.
The database is built upoen predicted T cell epitopes covering 124 proteins that are shared between the following
orthopoxvirus:
Virus | STRAIN | ACC | Genes |
Variola virus (VARV) major | Bangladesh-1975 | L22579 | 189 |
VARV major | India-1967 | NC_00161 | 197 |
VARV minor | Garcia-1966 | Y16780 | 206 |
Monkeypox virus, MPXV | Zaire-96-I-16 | NC_003310 | 191 |
Cowpox virus, CPXV | Brighton Red | AF482758 | 218 |
Vaccinia virus, VACV | Copenhagen | M35027 | 262 |
VACV | Tian Tan | AF095689 | 243 |
VACV | Ankara | U94848 | 157 |
The list of proteins that are equivalent between the selected orthopoxvirus can be obtained here
HLA
EPIPOX contains both CD4 and CD8 T cell epitopes that have been predicted using peptide-MHC I and II binding profiles, respectively .
In particular, we used 32 HLA I and 33 HLA-II specific matrices. Users can select single of combination of
HLA I molcules to retrieve the relevant T cell epitoptes. Here follow the list of HLA molecules in EPIPOX:
HLA I-restricted T cell epitopes | HLA-II restricted T cell epitopes |
HLA I | Epitopes | HLA II | Epitopes |
HLA_A0201 | 578 | DRB1_0102 | 1000 |
HLA_A0202 | 581 | DRB1_0301 | 982 |
HLA_A0203 | 598 | DRB1_0311 | 988 |
HLA_A0205 | 589 | DRB1_0401 | 975 |
HLA_A0206 | 592 | DRB1_0402 | 988 |
HLA_A0207 | 570 | DRB1_0404 | 978 |
HLA_A0301 | 577 | DRB1_0405 | 977 |
HLA_A0101 | 589 | DRB1_0406 | 944 |
HLA_A1101 | 575 | DRB1_0407 | 934 |
HLA_A2301 | 601 | DRB1_0408 | 969 |
HLA_A2402 | 601 | DRB1_0410 | 977 |
HLA_A2403 | 601 | DRB1_0801 | 1026 |
HLA_A2405 | 601 | DRB1_0802 | 1006 |
HLA_A2407 | 601 | DRB1_0804 | 1001 |
HLA_A3101 | 567 | DRB1_0806 | 995 |
HLA_A3301 | 579 | DRB1_0901 | 1022 |
HLA_A6801 | 578 | DRB1_1101 | 1014 |
HLA_A6802 | 589 | DRB1_1102 | 1008 |
HLA_B0702 | 595 | DRB1_1103 | 973 |
HLA_B1501 | 583 | DRB1_1104 | 1005 |
HLA_B1502 | 575 | DRB1_1106 | 1005 |
HLA_B2705 | 561 | DRB1_1201 | 955 |
HLA_B3501 | 595 | DRB1_1301 | 1013 |
HLA_B4402 | 585 | DRB1_1302 | 1042 |
HLA_B5101 | 573 | DRB1_1304 | 994 |
HLA_B5301 | 599 | DRB1_1305 | 1013 |
HLA_B5401 | 597 | DRB1_1501 | 1018 |
HLA_B5801 | 601 | DRB1_1502 | 1010 |
HLA_B0801 | 565 | DRB3_0101 | 960 |
HLA_C0102 | 571 | DRB3_0202 | 946 |
HLA_C0304 | 575 | DRB4_0101 | 977 |
HLA_C0702 | 584 | DRB5_0101 | 1034 |
| DRB1_0101 | 993 |
T cell epitopes can be queried by any of the listed HLA molecules.
SUPERTYPES
Supertypes are set of HLA molecules that have simililar peptide binding reperpertoires. In EPIPOX, we provide the
facility of identifying peptides that are predicted by 3 main supertypes
(A2, A3 and B7):
A2: A*0201, A*0202, A*0203, A*0205, A*0206, A*0207, A6802
A3: A*0301, A*1101, A*3101, A*3301, A*6801, A*6601
A24: B*0702, B*3501, B*5101, B*5102, B*5301, B*5401
The alleles belogning to these supertypes are present in 88 % of the population regardless of their ething groups. Selecting promiscous peptides
restricted by these 3 supertypes facilitate maximizaing the population coverage of vaccines with minimum number
of peptides. For more details check Reche & Reche, NAR, 2005: W138-42
LIMITING SEARCH RESULTS
T cell epitopes searches results in EPIPOX can be limited by several criteria, including
temporal expression of genes and features of antigens:
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